Excitonic States and Excitation Energy Transfer in Plant Light-Harvesting Complexes in Different Molecular Environments

Akhtar Parveen
Excitonic States and Excitation Energy Transfer in Plant Light-Harvesting Complexes in Different Molecular Environments.
Doctoral thesis (PhD), University of Szeged.
(2018) (Unpublished)

[thumbnail of Akhtar Thesis.pdf]
PDF (thesis)
Download (12MB) | Preview
[thumbnail of Akhtar Thesis Booklet ENG.pdf]
PDF (booklet)
Download (577kB) | Preview
[thumbnail of Akhtar Thesis Booklet HUN.pdf]
PDF (booklet)
Download (666kB) | Preview
[thumbnail of Akhtar Articles.zip] PDF (related own publications)
Download (9MB)

Abstract in foreign language

Light-harvesting complex II (LHCII), the main light-harvesting antenna complex of plants, is a functionally flexible and mobile component of the photosynthetic membrane. Excitonic interactions between chlorophylls in the complex are the basis for fast and efficient excitation energy transfer (EET). In addition to its light-harvesting function, LHCII plays regulatory roles, e.g. balancing the energy flow between Photosystem II and Photosystem I (PSI) and activating photoprotective non-photochemical quenching (NPQ). These functions are controlled by the innate structural flexibility of the complex and its intermolecular interactions in the thylakoid membranes. The general aim of this thesis work is to clarify the changes in the molecular and excitonic structure of LHCII that are incurred by interactions with its environment, and how these changes affect EET in the complex and in the thylakoid membrane. Using CD and anisotropic CD (ACD) spectroscopy, we have identified specific changes in the excitonic states in LHCII related to protein-protein interactions in aggregates or induced by detergents and. Time-resolve fluorescence showed that the molecular environment strongly affects the excitation lifetime, hence the light-harvesting function, of LHCII. Due to self-segregation, LHCII formed protein-dense domains in reconstituted membranes wherein fluorescence quenching occurred with a mechanism similar to NPQ in vivo. The dynamics of EET in LHCII was followed by ultrafast two-dimensional electronic spectroscopy (2DES), resolving simultaneous uphill and downhill energy transfer pathways. Exciton equilibration in the Chl a domain was found to occur on timescales up to 5 ps at physiological temperature. EET in plant PSI–LHCI and isolated PSI core complexes were observed for the first time by 2DES. A refined kinetic model was proposed, according to which primary charge separation in PSI occurs after full equilibration of the excitations in the core antenna, with an effective time constant of 3–4 ps. Long-distance EET between LHCIIs and between LHCII and PSI was detected in reconstituted membranes. LHCII acted as efficient antenna of PSI increasing its functional antenna size by up to 50% with minor loss of photochemical efficiency.

Item Type: Thesis (Doctoral thesis (PhD))
Creators: Akhtar Parveen
Hungarian title: A növényi fénybegyűjtő komplexek excitonállapotai és a gerjesztési energia vándorlása különböző molekuláris környezetekben
Position, academic title, institution
MTMT author ID
Lambrev Petar
senior research associate, PhD, Biological Research Centre of the Hungarian Academy of Sciences
Subjects: 01. Natural sciences > 01.06. Biological sciences
Divisions: Doctoral School of Biology
Discipline: Natural Sciences > Biology
Language: English
Date: 2018. November 21.
Uncontrolled Keywords: light-harvesting complex II, photosynthetic membranes, excitation energy transfer, time-resolved fluorescence, two-dimensional spectroscopy
Item ID: 9895
MTMT identifier of the thesis: 30617656
doi: https://doi.org/10.14232/phd.9895
Date Deposited: 2018. Jul. 26. 11:55
Last Modified: 2020. Jun. 30. 12:32
Depository no.: B 6454
URI: https://doktori.bibl.u-szeged.hu/id/eprint/9895
Defence/Citable status: Defended.

Actions (login required)

View Item View Item


Downloads per month over past year