Kovács Gergő
Rybp is required for neural differentiation of mouse embryonic stem cells.
Doctoral thesis (PhD), University of Szeged.
(2016)
(Unpublished)
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Abstract in foreign language
In current thesis I have investigated the function of the non-canonical PRC1 member, Rybp during neural lineage commitment. For this purpose, we utilized wild type and rybp null mutant ES cells and differentiated them in vitro into neural lineages. Our results showed that the formation of NSCs and NPCs was excessive in the rybp null mutant compared to the wild type, however they cannot form matured neural cell types properly. The absence of Rybp caused alteration in expression of key neural markers (Nestin, Tubb3, Gfap, Map2, Tau) and transcription factors (Sox2, Pax6, NeuroD1, NeuN, Olig2, Plagl1). The endogenous Plagl1 is one of the most downregulated genes suggesting a possible transcription circuit between Rybp, Plagl1 and other transcriptional factors. This study demonstrates the importance of Rybp in in vitro neural lineage specification.
Item Type: | Thesis (Doctoral thesis (PhD)) |
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Creators: | Kovács Gergő |
Hungarian title: | A polikomb fehérje, Rybp kulcsfontosságú az egér embrionális őssejtek neurális differenciációjához |
Supervisor(s): | Supervisor Position, academic title, institution MTMT author ID Pirity Melinda tudományos főmunkatárs, PhD, MTA SZBK Genetikai Intézet 10025270 |
Subjects: | 01. Natural sciences > 01.06. Biological sciences |
Divisions: | Doctoral School of Theoretical Medicine |
Discipline: | Natural Sciences > Biology |
Language: | English |
Date: | 2016. May 20. |
Item ID: | 2858 |
MTMT identifier of the thesis: | 3105066 |
doi: | https://doi.org/10.14232/phd.2858 |
Date Deposited: | 2016. Feb. 26. 15:17 |
Last Modified: | 2020. Apr. 29. 09:29 |
Depository no.: | B 6037 |
URI: | https://doktori.bibl.u-szeged.hu/id/eprint/2858 |
Defence/Citable status: | Defended. |
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