Rybp is required for neural differentiation of mouse embryonic stem cells

Kovács Gergő
Rybp is required for neural differentiation of mouse embryonic stem cells.
Doctoral thesis (PhD), University of Szeged.
(2016) (Unpublished)

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Abstract in foreign language

In current thesis I have investigated the function of the non-canonical PRC1 member, Rybp during neural lineage commitment. For this purpose, we utilized wild type and rybp null mutant ES cells and differentiated them in vitro into neural lineages. Our results showed that the formation of NSCs and NPCs was excessive in the rybp null mutant compared to the wild type, however they cannot form matured neural cell types properly. The absence of Rybp caused alteration in expression of key neural markers (Nestin, Tubb3, Gfap, Map2, Tau) and transcription factors (Sox2, Pax6, NeuroD1, NeuN, Olig2, Plagl1). The endogenous Plagl1 is one of the most downregulated genes suggesting a possible transcription circuit between Rybp, Plagl1 and other transcriptional factors. This study demonstrates the importance of Rybp in in vitro neural lineage specification.

Item Type: Thesis (Doctoral thesis (PhD))
Creators: Kovács Gergő
Hungarian title: A polikomb fehérje, Rybp kulcsfontosságú az egér embrionális őssejtek neurális differenciációjához
Supervisor(s):
Supervisor
Position, academic title, institution
MTMT author ID
Pirity Melinda
tudományos főmunkatárs, PhD, MTA SZBK Genetikai Intézet
10025270
Subjects: 01. Natural sciences > 01.06. Biological sciences
Divisions: Doctoral School of Theoretical Medicine
Discipline: Natural Sciences > Biology
Language: English
Date: 2016. May 20.
Item ID: 2858
MTMT identifier of the thesis: 3105066
doi: https://doi.org/10.14232/phd.2858
Date Deposited: 2016. Feb. 26. 15:17
Last Modified: 2020. Apr. 29. 09:29
Depository no.: B 6037
URI: https://doktori.bibl.u-szeged.hu/id/eprint/2858
Defence/Citable status: Defended.

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