Oláh Péter
High-throughput transcriptomic analysis of Pseudorabies virus.
Doctoral thesis (PhD), University of Szeged.
(2017)
(Unpublished)
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Abstract in foreign language
The whole-genome sequence of PRV strain Ka was determined by cutting-edge long-read sequencing in order to facilitate accurate transcriptome mapping and further epigenetic studies of the virus. Illumina short-read, high-throughput sequencing was used for the first time on lytic-infection PRV samples in order to create a single-base resolution transcriptome map, along with the detailed polyadenylation landscape of the virus by PA-Seq. In accordance with expectations, most of the viral genome was transcribed, with the exception of several small intergenic repetitive sequences, and loci in the large internal and terminal repeats. Among the findings are a novel polyadenylated lncRNA near the OriL origin of replication, and the single-base resolution mapping of 3′ UTRs across the viral genome. A number of genes exhibited alternative polyadenylation sites, while previously described splice sites were confirmed and expanded with a novel alternative splicing event in the key regulator ep0 gene.
| Item Type: | Thesis (Doctoral thesis (PhD)) |
|---|---|
| Creators: | Oláh Péter |
| Hungarian title: | A Pseudorabies vírus transzkriptomikai elemzése nagy áteresztőképességű módszerekkel |
| Supervisor(s): | Supervisor Position, academic title, institution MTMT author ID Boldogkői Zsolt tanszékvezető egyetemi tanár, habil., PhD, DSc, SZTE ÁOK Orvosi Biológiai Intézet 10012608 |
| Subjects: | 03. Medical and health sciences > 03.01. Basic medicine |
| Divisions: | Doctoral School of Interdisciplinary Sciences |
| Discipline: | Medicine > Theoretical Medicine |
| Language: | English |
| Date: | 2017. April 24. |
| Item ID: | 3538 |
| MTMT identifier of the thesis: | 3266861 |
| doi: | https://doi.org/10.14232/phd.3538 |
| Date Deposited: | 2017. Jan. 17. 16:14 |
| Last Modified: | 2020. May. 20. 08:46 |
| URI: | https://doktori.bibl.u-szeged.hu/id/eprint/3538 |
| Defence/Citable status: | Defended. |
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