Rybp is required for neural differentiation of mouse embryonic stem cells

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In current thesis I have investigated the function of the non-canonical PRC1 member, Rybp during neural lineage commitment. For this purpose, we utilized wild type and rybp null mutant ES cells and differentiated them in vitro into neural lineages. Our results showed that the formation of NSCs and NPCs was excessive in the rybp null mutant compared to the wild type, however they cannot form matured neural cell types properly. The absence of Rybp caused alteration in expression of key neural markers (Nestin, Tubb3, Gfap, Map2, Tau) and transcription factors (Sox2, Pax6, NeuroD1, NeuN, Olig2, Plagl1). The endogenous Plagl1 is one of the most downregulated genes suggesting a possible transcription circuit between Rybp, Plagl1 and other transcriptional factors. This study demonstrates the importance of Rybp in in vitro neural lineage specification.

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